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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HMGA2 Polyclonal antibody

HMGA2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20795

Print datasheet

Synonyms

BABL, high mobility group AT hook 2, HMGA2, HMGI C, HMGIC, LIPO, STQTL9

验证数据展示

  • IF Staining of A549 using CL488-20795

    IF Staining of A549 using CL488-20795

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 HMGA2 antibody (CL488-20795) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy HMGA2 Ready-To-Use IHC Kit. HMGA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20795-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20795 targets HMGA2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen HMGA2 fusion protein Ag14588 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name high mobility group AT-hook 2
Synonyms BABL, high mobility group AT hook 2, HMGA2, HMGI C, HMGIC, LIPO, STQTL9
Calculated Molecular Weight 108 aa, 12 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberNM_003483
Gene Symbol HMGA2
Gene ID (NCBI) 8091
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52926
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (PMID: 18202751,19551524). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (18425117). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (19551524). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (PMID: 20228781). The calcualted molecular weight of HMGA2 is 12 kDa, but modified HMGA2 is about 18-20 kDa. (PMID: 18505920 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HMGA2 antibody CL488-20795Download protocol
Standard Protocols
Click here to view our Standard Protocols