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HMGCL Recombinant antibody

HMGCL Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242224G10

Cat no : 84769-1-RR

Print datasheet

Synonyms

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224G10

验证数据展示

  • WB analysis using 84769-1-RR

    WB analysis using 84769-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84769-1-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84769-1-RR

    Affinity and Kinetic Characterization of 84769-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84769-1-RR against Human HMGCL were performed. The affinity constant is 0.408 nM.

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, A431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84769-1-RR targets HMGCL in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen HMGCL fusion protein Ag10426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Synonyms HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224G10
Calculated Molecular Weight 325 aa, 34 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC010570
Gene Symbol HMGCL
Gene ID (NCBI) 3155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.

实验方案

Product Specific Protocols
WB protocol for HMGCL antibody 84769-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols