验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84769-1-RR targets HMGCL in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | HMGCL fusion protein Ag10426 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase |
| 别名 | HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224G10 |
| 计算分子量 | 325 aa, 34 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC010570 |
| 基因名称 | HMGCL |
| Gene ID (NCBI) | 3155 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35914 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HMGCL antibody 84769-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |