验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
11695-1-AP targets HMGN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | HMGN1 fusion protein Ag2315 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | high-mobility group nucleosome binding domain 1 |
| 别名 | Non-histone chromosomal protein HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1 |
| 计算分子量 | 100 aa, 11 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC023984 |
| 基因名称 | HMGN1 |
| Gene ID (NCBI) | 3150 |
| RRID | AB_2248375 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05114 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HMGN1 (high-mobility group nucleosome-binding protein 1) is a member of the HMG superfamily of nonhistone chromatin-binding proteins, which which regulate chromatin structure and gene transcription. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. It may participate in the process which maintains transcribable genes in an unique chromatin conformation, and inhibit the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 expressed abundantly
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HMGN1 antibody 11695-1-AP | Download protocol |
| IHC protocol for HMGN1 antibody 11695-1-AP | Download protocol |
| IF protocol for HMGN1 antibody 11695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Med High-mobility group nucleosome-binding protein 1 acts as an alarmin and is critical for lipopolysaccharide-induced immune responses. | ||
Nat Commun Alarmin-painted exosomes elicit persistent antitumor immunity in large established tumors in mice. | ||
Cancer Res The alarmin HMGN1 contributes to anti-tumor immunity and is a potent immunoadjuvant. | ||
Theranostics Alarmin augments the antitumor immunity of lentiviral vaccine in ectopic, orthotopic and autochthonous hepatocellular carcinoma mice. | ||
J Proteomics Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection. | ||
PeerJ PM2.5 exposure aggravates kidney damage by facilitating the lipid metabolism disorder in diabetic mice |
