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验证数据展示

  • IF Staining of HepG2 using CL488-11695

    IF Staining of HepG2 using CL488-11695

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HMGN1 antibody (CL488-11695) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-11695

    FC experiment of HepG2 using CL488-11695

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Hmgn1 Polyclonal Antibody (CL488-11695)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated HMGN1 Polyclonal antibody

HMGN1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11695

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Non-histone chromosomal protein HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HMGN1 Ready-To-Use IHC Kit. HMGN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11695-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11695 targets HMGN1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 HMGN1 fusion protein Ag2315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 high-mobility group nucleosome binding domain 1
别名 Non-histone chromosomal protein HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1
计算分子量 100 aa, 11 kDa
观测分子量 17 kDa
GenBank蛋白编号BC023984
基因名称 HMGN1
Gene ID (NCBI) 3150
RRIDAB_3672507
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05114
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGN1 (high-mobility group nucleosome-binding protein 1) is a member of the HMG superfamily of nonhistone chromatin-binding proteins, which which regulate chromatin structure and gene transcription. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. It may participate in the process which maintains transcribable genes in an unique chromatin conformation, and inhibit the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 expressed abundantly

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HMGN1 antibody CL488-11695Download protocol
FC protocol for CL Plus 488 HMGN1 antibody CL488-11695Download protocol
Standard Protocols
Click here to view our Standard Protocols