验证数据展示
经过测试的应用
Positive WB detected in | MDCK cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84684-4-RR targets HNF1B in WB, ELISA applications and shows reactivity with human, canine samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, canine |
免疫原 | HNF1B fusion protein Ag3232 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | HNF1 homeobox B |
别名 | TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta |
计算分子量 | 557 aa, 61 kDa |
观测分子量 | 58-65 kDa |
GenBank蛋白编号 | BC017714 |
基因名称 | HNF1B |
Gene ID (NCBI) | 6928 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P35680 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.
实验方案
Product Specific Protocols | |
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WB protocol for HNF1B antibody 84684-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |