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HNF1B Recombinant antibody

HNF1B Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242120A9

Cat no : 84684-4-RR

Print datasheet

Synonyms

TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta

验证数据展示

  • WB analysis of MDCK using 84684-4-RR

    WB analysis of MDCK using 84684-4-RR

    MDCK cells were subjected to SDS PAGE followed by western blot with 84684-4-RR (HNF1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84684-4-RR

    Affinity and Kinetic Characterization of 84684-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84684-4-RR against Human HNF1B were performed. The affinity constant is 0.232 nM.

经过测试的应用

Positive WB detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy HNF1B Ready-To-Use IHC Kit. HNF1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84684-4-RR targets HNF1B in WB, ELISA applications and shows reactivity with human, canine samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, canine
Immunogen HNF1B fusion protein Ag3232 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HNF1 homeobox B
Synonyms TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta
Calculated Molecular Weight 557 aa, 61 kDa
Observed Molecular Weight 58-65 kDa
GenBank Accession NumberBC017714
Gene Symbol HNF1B
Gene ID (NCBI) 6928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP35680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.

实验方案

Product Specific Protocols
WB protocol for HNF1B antibody 84684-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols