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HNF1B Recombinant antibody

HNF1B Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242120H8

Cat no : 84684-5-RR

Print datasheet

Synonyms

TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta

验证数据展示

  • IF Staining of HepG2 using 84684-5-RR

    IF Staining of HepG2 using 84684-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of Caco-2 using 84684-5-RR

    IF Staining of Caco-2 using 84684-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84684-5-RR

    Affinity and Kinetic Characterization of 84684-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84684-5-RR against Human HNF1B were performed. The affinity constant is 13.5 nM.

经过测试的应用

Positive IF/ICC detected inHepG2 cells, Caco-2 cells
Planning an IHC experiment? We recommend our IHCeasy HNF1B Ready-To-Use IHC Kit. HNF1B primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84684-5-RR targets HNF1B in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HNF1B fusion protein Ag3232 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HNF1 homeobox B
Synonyms TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta
Calculated Molecular Weight 557 aa, 61 kDa
GenBank Accession NumberBC017714
Gene Symbol HNF1B
Gene ID (NCBI) 6928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP35680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.

实验方案

Product Specific Protocols
IF protocol for HNF1B antibody 84684-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols