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HNMT Recombinant antibody

HNMT Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242159E3

Cat no : 84777-3-RR

Print datasheet

Synonyms

HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase, EC:2.1.1.8

验证数据展示

  • WB analysis using 84777-3-RR

    WB analysis using 84777-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84777-3-RR (HNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84777-3-RR

    Affinity and Kinetic Characterization of 84777-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84777-3-RR against Human HNMT were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84777-3-RR targets HNMT in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen HNMT fusion protein Ag2462 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histamine N-methyltransferase
Synonyms HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase, EC:2.1.1.8
Calculated Molecular Weight 292 aa, 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC020677
Gene Symbol HNMT
Gene ID (NCBI) 3176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50135
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.

实验方案

Product Specific Protocols
WB protocol for HNMT antibody 84777-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols