验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84777-3-RR targets HNMT in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | HNMT fusion protein Ag2462 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | histamine N-methyltransferase |
别名 | HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase, EC:2.1.1.8 |
计算分子量 | 292 aa, 33 kDa |
观测分子量 | 30-33 kDa |
GenBank蛋白编号 | BC020677 |
基因名称 | HNMT |
Gene ID (NCBI) | 3176 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P50135 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.
实验方案
Product Specific Protocols | |
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WB protocol for HNMT antibody 84777-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |