验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, Jurkat cells |
| Positive IHC detected in | human stomach tissue, human colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse colon tissue |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy HNRNPA3 Ready-To-Use IHC Kit. HNRNPA3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25142-1-AP targets HNRNPA3 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig, monkey |
| 免疫原 | HNRNPA3 fusion protein Ag18248 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heterogeneous nuclear ribonucleoprotein A3 |
| 别名 | HNRPA3, HNRNPA 3, hnRNP A3, FBRNP |
| 计算分子量 | 378 aa, 40 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC113470 |
| 基因名称 | HNRNPA3 |
| Gene ID (NCBI) | 220988 |
| RRID | AB_2879921 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51991 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HNRNPA3 also named as hnRNP A3 or heterogeneous nuclear ribonucleoprotein A3 is a 378 amino acid protein, which contains 2 RRM domains and localizes in nucleus. HNRNPA3 is a component of the spliceosome C complex and play a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HNRNPA3 antibody 25142-1-AP | Download protocol |
| IHC protocol for HNRNPA3 antibody 25142-1-AP | Download protocol |
| IF protocol for HNRNPA3 antibody 25142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism. | ||
Arterioscler Thromb Vasc Biol Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity. | ||
Neurobiol Dis ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity. | ||
Sci China Life Sci Identification of mecciRNAs and their roles in the mitochondrial entry of proteins. | ||
Mediators Inflamm Integrated Functional Analysis of the Nuclear Proteome of Classically and Alternatively Activated Macrophages. |
