验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy HNRNPA3 Ready-To-Use IHC Kit. HNRNPA3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25142-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-25142 targets HNRNPA3 in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | HNRNPA3 fusion protein Ag18248 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heterogeneous nuclear ribonucleoprotein A3 |
| 别名 | 2610510D13Rik, D10S102, FBRNP, hnRNP A3, HNRNPA3, HNRPA3 |
| 计算分子量 | 378 aa, 40 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC113470 |
| 基因名称 | HNRNPA3 |
| Gene ID (NCBI) | 220988 |
| RRID | AB_3084088 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51991 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HNRNPA3 also named as hnRNP A3 or heterogeneous nuclear ribonucleoprotein A3 is a 378 amino acid protein, which contains 2 RRM domains and localizes in nucleus. HNRNPA3 is a component of the spliceosome C complex and play a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HNRNPA3 antibody CL488-25142 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |