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验证数据展示

  • WB analysis using 84207-1-RR (same clone as 84207-1-PBS)

    WB analysis using 84207-1-RR (same clone as 84207-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84207-1-RR (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

    WB analysis of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

    WB result of HNRNPAB antibody (84207-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPAB transfected HeLa cells. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

    IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

    IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 84207-1-RR (same clone as 84207-1-PBS)

    FC experiment of Jurkat using 84207-1-RR (same clone as 84207-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01139-1

    Cytometric bead array standard curve of MP01139-1

    Cytometric bead array standard curve of MP01139-1, HNRNPAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84207-1-PBS. Detection antibody: 84207-2-PBS. Standard: Ag35010. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01139-2

    Cytometric bead array standard curve of MP01139-2

    Cytometric bead array standard curve of MP01139-2, HNRNPAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84207-1-PBS. Detection antibody: 84207-3-PBS. Standard: Ag35010. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84207-1-PBS

    Affinity and Kinetic Characterization of 84207-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 84207-1-PBS against Human HNRNPAB was performed. The affinity constant is 2.01 nM.

  • Affinity and Kinetic Characterization of 84207-1-RR

    Affinity and Kinetic Characterization of 84207-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84207-1-RR against Human HNRNPAB were performed. The affinity constant is 1.07 nM.

  • Featured Product
  • KD/KO验证

HNRNPAB Recombinant antibody, PBS Only (Capture)

HNRNPAB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84207-1-PBS

产品说明书

CloneNo. 241462B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

241462B1, ABBP 1, ABBP1, ABBP-1, APOBEC1-binding protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84207-1-PBS targets HNRNPAB as part of a matched antibody pair:

MP01139-1: 84207-1-PBS capture and 84207-2-PBS detection (validated in Cytometric bead array)

MP01139-2: 84207-1-PBS capture and 84207-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 HNRNPAB fusion protein Ag35010 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein A/B
别名 241462B1, ABBP 1, ABBP1, ABBP-1, APOBEC1-binding protein 1
计算分子量36 kDa
观测分子量36-42 kDa
GenBank蛋白编号BC036708
基因名称 HNRNPAB
Gene ID (NCBI) 3182
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99729
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).