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验证数据展示

  • WB analysis using 84207-1-RR

    WB analysis using 84207-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84207-1-RR (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 84207-1-RR

    WB analysis of HeLa using 84207-1-RR

    WB result of HNRNPAB antibody (84207-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPAB transfected HeLa cells.

  • IF Staining of HeLa using 84207-1-RR

    IF Staining of HeLa using 84207-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84207-1-RR

    IF Staining of HeLa using 84207-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

  • FC experiment of Jurkat using 84207-1-RR

    FC experiment of Jurkat using 84207-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84207-1-RR

    Affinity and Kinetic Characterization of 84207-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84207-1-RR against Human HNRNPAB were performed. The affinity constant is 1.07 nM.

  • Featured Product
  • KD/KO验证

HNRNPAB Recombinant antibody

HNRNPAB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84207-1-RR

产品说明书

CloneNo. 241462B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, ABBP1, ABBP 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, HEK-293T cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84207-1-RR targets HNRNPAB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 HNRNPAB fusion protein Ag35010 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein A/B
别名 hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, ABBP1, ABBP 1
计算分子量36 kDa
观测分子量36-42 kDa
GenBank蛋白编号BC036708
基因名称 HNRNPAB
Gene ID (NCBI) 3182
RRIDAB_3671763
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).

实验方案

Product Specific Protocols
WB protocol for HNRNPAB antibody 84207-1-RRDownload protocol
IF protocol for HNRNPAB antibody 84207-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols