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验证数据展示

  • WB analysis of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    WB analysis of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    WB result of HNRNPC antibody (11760-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPC transfected HeLa cells. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • WB analysis using 11760-1-AP (same clone as 11760-1-PBS)

    WB analysis using 11760-1-AP (same clone as 11760-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    IP experiment of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    IP result of anti-HNRNPC (IP:11760-1-AP, 4ug; Detection:11760-1-AP 1:10000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 11760-1-AP (same clone as 11760-1-PBS)

    IHC staining of mouse colon using 11760-1-AP (same clone as 11760-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPC antibody included
  • IF Staining of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    IF Staining of HeLa using 11760-1-AP (same clone as 11760-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (11760-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11760-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HNRNPC Polyclonal antibody, PBS Only

HNRNPC Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11760-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HNRNPC Ready-To-Use IHC Kit. HNRNPC primary antibody included.

产品信息

11760-1-PBS targets HNRNPC in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HNRNPC fusion protein Ag2356 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein C (C1/C2)
别名
计算分子量 32 kDa
观测分子量 32 kDa, 36-40 kDa
GenBank蛋白编号BC003394
基因名称 HNRNPC
Gene ID (NCBI) 3183
RRIDAB_2117500
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07910
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.