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  • KD/KO Validated

HNRNPC Polyclonal antibody

HNRNPC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11760-1-AP

Print datasheet

Synonyms

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC



经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, K-562 cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse colon tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPC Ready-To-Use IHC Kit. HNRNPC primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11760-1-AP targets HNRNPC in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, IP, RIP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen HNRNPC fusion protein Ag2356 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein C (C1/C2)
Synonyms C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa, 36-40 kDa
GenBank Accession NumberBC003394
Gene Symbol HNRNPC
Gene ID (NCBI) 3183
RRIDAB_2117500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

实验方案

Product Specific Protocols
WB protocol for HNRNPC antibody 11760-1-APDownload protocol
IHC protocol for HNRNPC antibody 11760-1-APDownload protocol
IF protocol for HNRNPC antibody 11760-1-APDownload protocol
IP protocol for HNRNPC antibody 11760-1-APDownload protocol
FC protocol for HNRNPC antibody 11760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
humanWB

Nat Struct Mol Biol

Alternative polyadenylation by sequential activation of distal and proximal PolyA sites.

Authors - Peng Tang
humanWB

Adv Sci (Weinh)

m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia

Authors - Zijuan Wu
  • KD Validated
humanWB

Nucleic Acids Res

Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

Authors - Misbah Khan
humanWB

Clin Transl Med

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing

Authors - Qi Wen Chen