CoraLite® Plus 488-conjugated HNRNPD Monoclonal antibody
HNRNPD Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, pig, rabbit
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2G9E6
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HNRNPD Ready-To-Use IHC Kit. HNRNPD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68236-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68236 targets HNRNPD in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat, pig, rabbit |
| Immunogen | HNRNPD fusion protein Ag3458 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) |
| Synonyms | AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37 |
| Calculated Molecular Weight | 355 aa, 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC026015 |
| Gene Symbol | HNRNPD |
| Gene ID (NCBI) | 3184 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14103 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region (PMID:8578590,12704645). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HNRNPD antibody CL488-68236 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |