验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68236 targets HNRNPD in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat, pig, rabbit |
| 免疫原 | HNRNPD fusion protein Ag3458 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) |
| 别名 | AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37 |
| 计算分子量 | 355 aa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC026015 |
| 基因名称 | HNRNPD |
| Gene ID (NCBI) | 3184 |
| RRID | AB_3084452 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14103 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region (PMID:8578590,12704645). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HNRNPD antibody CL488-68236 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |