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验证数据展示

  • WB analysis using 67375-1-Ig

    WB analysis using 67375-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67375-1-Ig

    IHC staining of human breast cancer using 67375-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HNRNPH1 antibody included
  • IF Staining of HepG2 using 67375-1-Ig

    IF Staining of HepG2 using 67375-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67375-1-Ig (HNRNPH1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

HNRNPH1 Monoclonal antibody

HNRNPH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67375-1-Ig

产品说明书

CloneNo. 1A6C2
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, U2OS cells, HSC-T6 cells, 4T1 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPH1 Ready-To-Use IHC Kit. HNRNPH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67375-1-Ig targets HNRNPH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 HNRNPH1 fusion protein Ag6603 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein H1 (H)
别名 HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed
计算分子量 49 kDa
观测分子量 49-50 kDa
GenBank蛋白编号BC001348
基因名称 HNRNPH1
Gene ID (NCBI) 3187
RRIDAB_2882623
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31943
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

实验方案

Product Specific Protocols
WB protocol for HNRNPH1 antibody 67375-1-IgDownload protocol
IHC protocol for HNRNPH1 antibody 67375-1-IgDownload protocol
IF protocol for HNRNPH1 antibody 67375-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing

Authors - Tamara Vital
View Article
humanWB

J Cell Physiol

MACC1 enhances an oncogenic RNA splicing of IRAK1 through interacting with HNRNPH1 in lung adenocarcinoma

Authors - Shiqing Wang
View Article