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验证数据展示

  • IF Staining of HepG2 using CL488-67375

    IF Staining of HepG2 using CL488-67375

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HNRNPH1 antibody (CL488-67375, Clone: 1A6C2 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated HNRNPH1 Monoclonal antibody

HNRNPH1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67375

产品说明书

CloneNo. 1A6C2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

DKFZp686A15170, hnRNP H, hnRNPH, HNRNPH1, HNRPH, HNRPH1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPH1 Ready-To-Use IHC Kit. HNRNPH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67375-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67375 targets HNRNPH1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 HNRNPH1 fusion protein Ag6603 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein H1 (H)
别名 DKFZp686A15170, hnRNP H, hnRNPH, HNRNPH1, HNRPH, HNRPH1
计算分子量 49 kDa
观测分子量 49-50 kDa
GenBank蛋白编号BC001348
基因名称 HNRNPH1
Gene ID (NCBI) 3187
RRIDAB_2919460
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31943
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HNRNPH1 antibody CL488-67375Download protocol
Standard Protocols
Click here to view our Standard Protocols