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验证数据展示

  • WB analysis using 85078-1-RR (same clone as 85078-1-PBS)

    WB analysis using 85078-1-RR (same clone as 85078-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85078-1-RR (HNRNPR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85078-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85078-1-RR (same clone as 85078-1-PBS)

    IF Staining of HepG2 using 85078-1-RR (same clone as 85078-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPR antibody (85078-1-RR, Clone: 242734C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85078-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01784-1

    Cytometric bead array standard curve of MP01784-1

    Cytometric bead array standard curve of MP01784-1, HNRNPR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85078-1-PBS. Detection antibody: 85078-2-PBS. Standard: Ag30339. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 85078-1-RR

    Affinity and Kinetic Characterization of 85078-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85078-1-RR against Human HNRNPR were performed. The affinity constant is 0.545 nM.

HNRNPR Recombinant antibody, PBS Only (Capture)

HNRNPR Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85078-1-PBS

产品说明书

CloneNo. 242734C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

HNRPR, hnRNP R, 242734C8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85078-1-PBS targets HNRNPR as part of a matched antibody pair:

MP01784-1: 85078-1-PBS capture and 85078-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HNRNPR fusion protein Ag30339 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein R
别名 HNRPR, hnRNP R, 242734C8
计算分子量 71 kDa
观测分子量65-82 kDa
GenBank蛋白编号BC001449
基因名称 HNRNPR
Gene ID (NCBI) 10236
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43390
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)