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验证数据展示

  • WB analysis using 85078-1-RR

    WB analysis using 85078-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85078-1-RR (HNRNPR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85078-1-RR

    IF Staining of HepG2 using 85078-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPR antibody (85078-1-RR, Clone: 242734C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85078-1-RR

    Affinity and Kinetic Characterization of 85078-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85078-1-RR against Human HNRNPR were performed. The affinity constant is 0.545 nM.

HNRNPR Recombinant antibody

HNRNPR Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85078-1-RR

产品说明书

CloneNo. 242734C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

HNRPR, hnRNP R, 242734C8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, U2OS cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85078-1-RR targets HNRNPR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HNRNPR fusion protein Ag30339 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein R
别名 HNRPR, hnRNP R, 242734C8
计算分子量 71 kDa
观测分子量65-82 kDa
GenBank蛋白编号BC001449
基因名称 HNRNPR
Gene ID (NCBI) 10236
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43390
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)

实验方案

Product Specific Protocols
WB protocol for HNRNPR antibody 85078-1-RRDownload protocol
IF protocol for HNRNPR antibody 85078-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols