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CoraLite® Plus 488-conjugated HNRNPU Polyclonal antibody

HNRNPU Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14599

Print datasheet

Synonyms

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1

验证数据展示

  • IF Staining of HeLa using CL488-14599

    IF Staining of HeLa using CL488-14599

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HNRNPU antibody (CL488-14599) at dilution of 1:200, CL594-phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPU Ready-To-Use IHC Kit. HNRNPU primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14599-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14599 targets HNRNPU in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen HNRNPU fusion protein Ag6141 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Synonyms hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1
Calculated Molecular Weight 825 aa, 91 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC003621
Gene Symbol HNRNPU
Gene ID (NCBI) 3192
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00839
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HNRNPU antibody CL488-14599Download protocol
Standard Protocols
Click here to view our Standard Protocols