验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-10578 targets HNRNPUL1 in IF/ICC applications and shows reactivity with human, mouse samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse |
免疫原 | HNRNPUL1 fusion protein Ag0899 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | heterogeneous nuclear ribonucleoprotein U-like 1 |
别名 | E1B AP5, E1BAP5, FLJ12944, HNRNPUL1, HNRPUL1 |
计算分子量 | 120 kDa |
观测分子量 | 90-115 kDa |
GenBank蛋白编号 | BC002564 |
基因名称 | HNRNPUL1 |
Gene ID (NCBI) | 11100 |
RRID | AB_3672452 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BUJ2 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1-Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4 .
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 HNRNPUL1 antibody CL488-10578 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |