• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

    WB analysis of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

    WB result of HO-1/HMOX1 antibody (66743-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1/HMOX1 transfected HeLa cells. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

    IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

    IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IF Staining of mouse spleen using 66743-1-Ig (same clone as 66743-1-PBS)

    IF Staining of mouse spleen using 66743-1-Ig (same clone as 66743-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (66743-1-Ig, Clone: 2D10A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

    FC experiment of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HO-1/HMOX1 Monoclonal antibody (66743-1-Ig, Clone:2D10A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HO-1/HMOX1 Monoclonal antibody, PBS Only

HO-1/HMOX1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 66743-1-PBS

产品说明书

CloneNo. 2D10A5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.

产品信息

66743-1-PBS targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 HO-1/HMOX1 fusion protein Ag21296 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 heme oxygenase (decycling) 1
别名 HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC001491
基因名称 HO-1
Gene ID (NCBI) 3162
RRIDAB_2882091
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09601
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).