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  • Featured Product
  • KD/KO Validated

HO-1/HMOX1 Monoclonal antibody, PBS Only

HO-1/HMOX1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D10A5

Cat no : 66743-1-PBS

Print datasheet

Synonyms

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

验证数据展示

  • WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

    WB analysis of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

    WB result of HO-1/HMOX1 antibody (66743-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1/HMOX1 transfected HeLa cells. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

    IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

    IHC staining of human liver cancer using 66743-1-Ig (same clone as 66743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IF Staining of mouse spleen using 66743-1-Ig (same clone as 66743-1-PBS)

    IF Staining of mouse spleen using 66743-1-Ig (same clone as 66743-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (66743-1-Ig, Clone: 2D10A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

    FC experiment of HeLa using 66743-1-Ig (same clone as 66743-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HO-1/HMOX1 Monoclonal antibody (66743-1-Ig, Clone:2D10A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

  • WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    WB analysis using 66743-1-Ig (same clone as 66743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66743-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.

产品信息

66743-1-PBS targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen HO-1/HMOX1 fusion protein Ag21296 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name heme oxygenase (decycling) 1
Synonyms HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001491
Gene Symbol HO-1
Gene ID (NCBI) 3162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09601
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).