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HO-1/HMOX1 Monoclonal antibody
HO-1/HMOX1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig, rabbit and More (1)
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (3)
Conjugate
Unconjugated
135
CloneNo.
2D10A5
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, A549 cells, pig spleen tissue, HepG2 cells, HeLa cells, HSC-T6 cells, rat liver tissue, rat spleen tissue, pig liver tissue, rabbit liver tissue |
Positive IHC detected in | human liver cancer tissue, human renal cell carcinoma tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse spleen tissue, rat liver tissue |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66743-1-Ig targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat, pig, rabbit |
Cited Reactivity | human, mouse, rat, pig, sheep |
Immunogen | HO-1/HMOX1 fusion protein Ag21296 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | heme oxygenase (decycling) 1 |
Synonyms | HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1 |
Calculated Molecular Weight | 33 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC001491 |
Gene Symbol | HO-1 |
Gene ID (NCBI) | 3162 |
RRID | AB_2882091 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P09601 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
IHC protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
IF protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Healthc Mater Bioinspired Multifunctional Black Phosphorus Hydrogel with Antibacterial and Antioxidant Properties: A Stepwise Countermeasure for Diabetic Skin Wound Healing. | ||
Biomaterials MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system. | ||
Oncogene The deubiquitinase USP7 regulates oxidative stress through stabilization of HO-1. | ||
EMBO Rep Eicosapentaenoic acid induces macrophage Mox polarization to prevent diabetic cardiomyopathy | ||
Cell Biosci Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth | ||
Oxid Med Cell Longev Xuesaitong Combined with Dexmedetomidine Improves Cerebral Ischemia-Reperfusion Injury in Rats by Activating Keap1/Nrf2 Signaling and Mitophagy in Hippocampal Tissue |