• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of human liver using CL594-66743

    IF Staining of human liver using CL594-66743

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594-conjugated HO-1/HMOX1 antibody (CL594-66743, Clone: 2D10A5 ) at dilution of 1:100.

  • IF Staining of mouse spleen using CL594-66743

    IF Staining of mouse spleen using CL594-66743

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite®594 HO-1/HMOX1 antibody (CL594-66743, Clone: 2D10A5 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using CL594-66743

    FC experiment of HeLa using CL594-66743

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human HO-1/HMOX1 (CL594-66743, Clone:2D10A5) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated HO-1/HMOX1 Monoclonal antibody

HO-1/HMOX1 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-66743

产品说明书

CloneNo. 2D10A5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

IF-P, FC (Intra)

别名

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF-P detected inmouse spleen tissue, human liver tissue
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66743-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66743 targets HO-1/HMOX1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 HO-1/HMOX1 fusion protein Ag21296 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 heme oxygenase (decycling) 1
别名 HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC001491
基因名称 HO-1
Gene ID (NCBI) 3162
RRIDAB_2883623
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09601
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

实验方案

Product Specific Protocols
IF protocol for CL594 HO-1/HMOX1 antibody CL594-66743Download protocol
FC protocol for CL594 HO-1/HMOX1 antibody CL594-66743Download protocol
Standard Protocols
Click here to view our Standard Protocols