- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated HO-1/HMOX1 Monoclonal antibody
HO-1/HMOX1 Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
IF-P, FC (Intra) and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D10A5
验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver tissue, mouse spleen tissue |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66743-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66743 targets HO-1/HMOX1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF-P, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Immunogen | HO-1/HMOX1 fusion protein Ag21296 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | heme oxygenase (decycling) 1 |
| Synonyms | HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC001491 |
| Gene Symbol | HO-1 |
| Gene ID (NCBI) | 3162 |
| RRID | AB_2883379 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09601 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HO-1/HMOX1 antibody CL488-66743 | Download protocol |
| FC protocol for CL Plus 488 HO-1/HMOX1 antibody CL488-66743 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
