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Mouse HO-1/Hmox1 Recombinant antibody, PBS Only (Capture)

HO-1/Hmox1 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240236G4

Cat no : 83291-3-PBS

Print datasheet

Synonyms

Hmox1, HO-1, Hmox, Hemox, heme oxygenase (decycling) 1

验证数据展示

  • WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

    WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

    IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 83291-3-RR (same clone as 83291-3-PBS)

    FC experiment of NIH/3T3 using 83291-3-RR (same clone as 83291-3-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug HO-1/Hmox1 Recombinant antibody (83291-3-RR, Clone:240236G4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00318-2

    Cytometric bead array standard curve of MP00318-2

    Cytometric bead array standard curve of MP00318-2, MOUSE HO-1/Hmox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83291-3-PBS. Detection antibody: 83291-2-PBS. Standard: Eg0938. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83291-3-RR

    Affinity and Kinetic Characterization of 83291-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83291-3-RR against Mouse HO-1/Hmox1 were performed. The affinity constant is 1.23 nM.

产品信息

83291-3-PBS targets HO-1/Hmox1 as part of a matched antibody pair:

MP00318-2: 83291-3-PBS capture and 83291-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heme oxygenase (decycling) 1
Synonyms Hmox1, HO-1, Hmox, Hemox, heme oxygenase (decycling) 1
Calculated Molecular Weight33 kDa
GenBank Accession NumberNM_010442.2
Gene Symbol HO-1
Gene ID (NCBI) 15368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14901
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C