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验证数据展示

  • WB analysis using 68597-1-Ig

    WB analysis using 68597-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68597-1-Ig

    WB analysis using 68597-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 68597-1-Ig

    IF Staining of SH-SY5Y using 68597-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using HOMER1 antibody (68597-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

HOMER1 Monoclonal antibody

HOMER1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68597-1-Ig

产品说明书

CloneNo. 3B1B11

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Rabbit, Pig, Chicken

应用

WB, IF/ICC, ELISA

别名

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, HOMER1, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, LNCaP cells, Calu-3 cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, MCF-10A cells, MOLT-4 cells, rat brain tisue, chicken brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68597-1-Ig targets HOMER1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig, Chicken samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Pig, Chicken
免疫原 HOMER1 fusion protein Ag21302 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 homer homolog 1 (Drosophila)
别名 HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, HOMER1, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves 1
计算分子量 40 kDa
观测分子量 40-46 kDa
GenBank蛋白编号BC015502
基因名称 HOMER1
Gene ID (NCBI) 9456
RRIDAB_3085295
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86YM7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.

实验方案

Product Specific Protocols
WB protocol for HOMER1 antibody 68597-1-IgDownload protocol
IF protocol for HOMER1 antibody 68597-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols