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HOMER1 Monoclonal antibody, PBS Only

HOMER1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B1B11

Cat No : 68597-1-PBS

Print datasheet

Synonyms

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, HOMER1, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves 1

验证数据展示

  • WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

    WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

  • WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

    WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 68597-1-Ig (same clone as 68597-1-PBS)

    IF Staining of SH-SY5Y using 68597-1-Ig (same clone as 68597-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using HOMER1 antibody (68597-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

产品信息

68597-1-PBS targets HOMER1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Immunogen HOMER1 fusion protein Ag21302 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name homer homolog 1 (Drosophila)
Synonyms HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, HOMER1, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves 1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC015502
Gene Symbol HOMER1
Gene ID (NCBI) 9456
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86YM7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.