- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated HOMER1 Polyclonal antibody
HOMER1 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | PC-12 cells |
Positive FC (Intra) detected in | SH-SY5Y cells |
For other applications, we recommend the unconjugated version of this antibody, 12433-1-AP
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-12433 targets HOMER1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | HOMER1 fusion protein Ag3109 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | homer homolog 1 (Drosophila) |
Synonyms | HOMER1A, Homer-1, Homer protein homolog 1, homer homolog 1 (Drosophila), Homer 1 |
Calculated Molecular Weight | 40 kDa |
Observed Molecular Weight | 46-48 kDa |
GenBank Accession Number | BC015502 |
Gene Symbol | HOMER1 |
Gene ID (NCBI) | 9456 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86YM7 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 HOMER1 antibody CL488-12433 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |