验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HEK-293T cells, MCF-7 cells, rat kidney tissue, LnCaP cells |
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
11143-1-AP targets HOMER2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | HOMER2 fusion protein Ag1606 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | homer homolog 2 (Drosophila) |
别名 | Homer protein homolog 2, homer homolog 2 (Drosophila), HOMER 2, Cupidin, CPD |
计算分子量 | 41 kDa |
观测分子量 | 41 kDa |
GenBank蛋白编号 | BC012109 |
基因名称 | HOMER2 |
Gene ID (NCBI) | 9455 |
RRID | AB_2877747 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NSB8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HOMER2 (Homer Scaffold Protein 2), a postsynaptic density scaffolding protein, is essential for normal hearing in humans and mice(PMID: 25816005). HOMER2 negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway(Uniprot). HOMER2 belongs to the homer family of dendritic proteins which are immediate early gene products who bind to group 1 metabotropic glutamate receptors (mGluRs), proteins associated to triggering intracellular calcium release. Additionally, HOMER2 connects mGluRs to the inositol triphosphate receptors (IP3R).
实验方案
Product Specific Protocols | |
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WB protocol for HOMER2 antibody 11143-1-AP | Download protocol |
IHC protocol for HOMER2 antibody 11143-1-AP | Download protocol |
IF protocol for HOMER2 antibody 11143-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |