验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HEK-293T cells, MCF-7 cells, rat kidney tissue, LnCaP cells |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11143-1-AP targets HOMER2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | HOMER2 fusion protein Ag1606 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | homer homolog 2 (Drosophila) |
| Synonyms | Homer protein homolog 2, homer homolog 2 (Drosophila), HOMER 2, Cupidin, CPD |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC012109 |
| Gene Symbol | HOMER2 |
| Gene ID (NCBI) | 9455 |
| RRID | AB_2877747 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NSB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HOMER2 (Homer Scaffold Protein 2), a postsynaptic density scaffolding protein, is essential for normal hearing in humans and mice(PMID: 25816005). HOMER2 negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway(Uniprot). HOMER2 belongs to the homer family of dendritic proteins which are immediate early gene products who bind to group 1 metabotropic glutamate receptors (mGluRs), proteins associated to triggering intracellular calcium release. Additionally, HOMER2 connects mGluRs to the inositol triphosphate receptors (IP3R).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HOMER2 antibody 11143-1-AP | Download protocol |
| IHC protocol for HOMER2 antibody 11143-1-AP | Download protocol |
| IF protocol for HOMER2 antibody 11143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
