HOMER2 Polyclonal antibody

HOMER2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11143-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

Homer protein homolog 2, homer homolog 2 (Drosophila), HOMER 2, Cupidin, CPD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293T cells, MCF-7 cells, rat kidney tissue, LnCaP cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11143-1-AP targets HOMER2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 HOMER2 fusion protein Ag1606 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homer homolog 2 (Drosophila)
别名 Homer protein homolog 2, homer homolog 2 (Drosophila), HOMER 2, Cupidin, CPD
计算分子量 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC012109
基因名称 HOMER2
Gene ID (NCBI) 9455
RRIDAB_2877747
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HOMER2 (Homer Scaffold Protein 2), a postsynaptic density scaffolding protein, is essential for normal hearing in humans and mice(PMID: 25816005). HOMER2 negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway(Uniprot). HOMER2 belongs to the homer family of dendritic proteins which are immediate early gene products who bind to group 1 metabotropic glutamate receptors (mGluRs), proteins associated to triggering intracellular calcium release. Additionally, HOMER2 connects mGluRs to the inositol triphosphate receptors (IP3R).

实验方案

Product Specific Protocols
WB protocol for HOMER2 antibody 11143-1-APDownload protocol
IHC protocol for HOMER2 antibody 11143-1-APDownload protocol
IF protocol for HOMER2 antibody 11143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Psychiatry

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Authors - Yue Qin
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