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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of human testis using 84003-5-RR (same clone as 84003-5-PBS)

    WB analysis of human testis using 84003-5-RR (same clone as 84003-5-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 84003-5-RR (HORMAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84003-5-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84003-5-RR (same clone as 84003-5-PBS)

    FC experiment of A549 using 84003-5-RR (same clone as 84003-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug HORMAD1 Recombinant antibody (84003-5-RR, Clone:241117E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84003-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84003-5-RR

    Affinity and Kinetic Characterization of 84003-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84003-5-RR against Human HORMAD1 were performed. The affinity constant is 2.32 nM.

HORMAD1 Recombinant antibody, PBS Only

HORMAD1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84003-5-PBS

产品说明书

CloneNo. 241117E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84003-5-PBS targets HORMAD1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 HORMAD1 fusion protein Ag4914 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HORMA domain containing 1
别名 NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3
计算分子量 45 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC047406
基因名称 HORMAD1
Gene ID (NCBI) 84072
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ86X24
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].