• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HORMAD1 Recombinant antibody, PBS Only

HORMAD1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241117E3

Cat no : 84003-5-PBS

Print datasheet

Synonyms

NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3

验证数据展示

  • WB analysis of human testis using 84003-5-RR (same clone as 84003-5-PBS)

    WB analysis of human testis using 84003-5-RR (same clone as 84003-5-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 84003-5-RR (HORMAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84003-5-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84003-5-RR (same clone as 84003-5-PBS)

    FC experiment of A549 using 84003-5-RR (same clone as 84003-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug HORMAD1 Recombinant antibody (84003-5-RR, Clone:241117E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84003-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84003-5-RR

    Affinity and Kinetic Characterization of 84003-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84003-5-RR against Human HORMAD1 were performed. The affinity constant is 2.32 nM.

产品信息

84003-5-PBS targets HORMAD1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen HORMAD1 fusion protein Ag4914 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HORMA domain containing 1
Synonyms NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC047406
Gene Symbol HORMAD1
Gene ID (NCBI) 84072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86X24
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].