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验证数据展示

  • WB analysis of human testis using 84003-5-RR

    WB analysis of human testis using 84003-5-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 84003-5-RR (HORMAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 84003-5-RR

    FC experiment of A549 using 84003-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug HORMAD1 Recombinant antibody (84003-5-RR, Clone:241117E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84003-5-RR

    Affinity and Kinetic Characterization of 84003-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84003-5-RR against Human HORMAD1 were performed. The affinity constant is 2.32 nM.

HORMAD1 Recombinant antibody

HORMAD1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84003-5-RR
CloneNo. 241117E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84003-5-RR targets HORMAD1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen HORMAD1 fusion protein Ag4914 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HORMA domain containing 1
Synonyms NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46, 241117E3
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC047406
Gene Symbol HORMAD1
Gene ID (NCBI) 84072
RRIDAB_3671574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86X24
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].

实验方案

Product Specific Protocols
WB protocol for HORMAD1 antibody 84003-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols