验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy HPGD Ready-To-Use IHC Kit. HPGD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66798-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66798 targets HPGD in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | HPGD fusion protein Ag1499 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | hydroxyprostaglandin dehydrogenase 15-(NAD) |
| 别名 | 15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 25-28 kDa |
| GenBank蛋白编号 | BC018986 |
| 基因名称 | HPGD |
| Gene ID (NCBI) | 3248 |
| RRID | AB_2920040 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15428 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 HPGD antibody CL594-66798 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |