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  • Featured Product
  • KD/KO Validated

HPRT1 Monoclonal antibody, PBS Only

HPRT1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1C3D1

Cat No : 67518-1-PBS

Print datasheet

Synonyms

HGPRT, HGPRTase, HPRT, HPRT1

验证数据展示

  • WB analysis using 67518-1-Ig (same clone as 67518-1-PBS)

    WB analysis using 67518-1-Ig (same clone as 67518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67518-1-Ig (HPRT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. HPRT1 deficient cell line Sp2/0 was served as a negative control. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67518-1-Ig (same clone as 67518-1-PBS)

    IF Staining of HeLa using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67518-1-Ig (HPRT1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included
  • WB analysis of HEK-293 using 67518-1-Ig (same clone as 67518-1-PBS)

    WB analysis of HEK-293 using 67518-1-Ig (same clone as 67518-1-PBS)

    WB result of HPRT1 antibody (67518-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.

产品信息

67518-1-PBS targets HPRT1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen HPRT1 fusion protein Ag7044 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hypoxanthine phosphoribosyltransferase 1
Synonyms HGPRT, HGPRTase, HPRT, HPRT1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC000578
Gene Symbol HPRT1
Gene ID (NCBI) 3251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00492
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HPRT1, also named as HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)