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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 67518-1-Ig (same clone as 67518-1-PBS)

    WB analysis using 67518-1-Ig (same clone as 67518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67518-1-Ig (HPRT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. HPRT deficient cell line Sp2/0 was served as a negative control. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    IHC staining of mouse liver using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    IHC staining of human endometrial cancer using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IF Staining of HeLa using 67518-1-Ig (same clone as 67518-1-PBS)

    IF Staining of HeLa using 67518-1-Ig (same clone as 67518-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HPRT antibody (67518-1-Ig, Clone: 1C3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67518-1-Ig (same clone as 67518-1-PBS)

    WB analysis of HEK-293 using 67518-1-Ig (same clone as 67518-1-PBS)

    WB result of HPRT antibody (67518-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT transfected HEK-293 cells. This data was developed using the same antibody clone with 67518-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HPRT Monoclonal antibody, PBS Only

HPRT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67518-1-PBS

Print datasheet

CloneNo. 1C3D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRTase, HGPRT, EC:2.4.2.8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.

产品信息

67518-1-PBS targets HPRT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HPRT fusion protein Ag7044 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hypoxanthine phosphoribosyltransferase 1
Synonyms HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRTase, HGPRT, EC:2.4.2.8
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC000578
Gene Symbol HPRT1
Gene ID (NCBI) 3251
RRIDAB_2882738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00492
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)