• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67518-1-Ig

    WB analysis using 67518-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67518-1-Ig (HPRT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. HPRT deficient cell line Sp2/0 was served as a negative control.

  • IHC staining of mouse liver using 67518-1-Ig

    IHC staining of mouse liver using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of mouse liver using 67518-1-Ig

    IHC staining of mouse liver using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of human endometrial cancer using 67518-1-Ig

    IHC staining of human endometrial cancer using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IHC staining of human endometrial cancer using 67518-1-Ig

    IHC staining of human endometrial cancer using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IF Staining of HeLa using 67518-1-Ig

    IF Staining of HeLa using 67518-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HPRT antibody (67518-1-Ig, Clone: 1C3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • WB analysis of HEK-293 using 67518-1-Ig

    WB analysis of HEK-293 using 67518-1-Ig

    WB result of HPRT antibody (67518-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

HPRT Monoclonal antibody

HPRT Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67518-1-Ig

产品说明书

CloneNo. 1C3D1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRTase, HGPRT, EC:2.4.2.8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human colon cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67518-1-Ig targets HPRT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HPRT fusion protein Ag7044 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hypoxanthine phosphoribosyltransferase 1
别名 HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRTase, HGPRT, EC:2.4.2.8
计算分子量 25 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC000578
基因名称 HPRT1
Gene ID (NCBI) 3251
RRIDAB_2882738
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00492
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)

实验方案

Product Specific Protocols
WB protocol for HPRT antibody 67518-1-IgDownload protocol
IHC protocol for HPRT antibody 67518-1-IgDownload protocol
IF protocol for HPRT antibody 67518-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols