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CoraLite® Plus 488-conjugated HPRT1 Monoclonal antibody
HPRT1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C3D1
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67518-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67518 targets HPRT1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | HPRT1 fusion protein Ag7044 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | hypoxanthine phosphoribosyltransferase 1 |
Synonyms | HGPRT, HGPRTase, HPRT, HPRT1 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 28 kDa |
GenBank Accession Number | BC000578 |
Gene Symbol | HPRT1 |
Gene ID (NCBI) | 3251 |
RRID | AB_2919488 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P00492 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HPRT1, also named as HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 HPRT1 antibody CL488-67518 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |