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CoraLite® Plus 488-conjugated HPSE Monoclonal antibody

HPSE Monoclonal Antibody for IF/ICC
Cat No. CL488-66226

产品说明书

CloneNo. 1D8B8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66226-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66226 targets HPSE in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 HPSE fusion protein Ag10067 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heparanase
别名 Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1
计算分子量 543 aa, 61 kDa
观测分子量 50 kDa
GenBank蛋白编号BC051321
基因名称 HPSE
Gene ID (NCBI) 10855
RRIDAB_2883289
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y251
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HPSE antibody CL488-66226Download protocol
Standard Protocols
Click here to view our Standard Protocols
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