验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
12930-1-AP targets HRSP12 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | HRSP12 fusion protein Ag3628 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heat-responsive protein 12 |
| 别名 | heat responsive protein 12, HRSP12, P14.5, PSP, Ribonuclease UK114, UK114, UK114 antigen homolog |
| 计算分子量 | 14.5 kDa |
| 观测分子量 | 14.5 kDa |
| GenBank蛋白编号 | BC010280 |
| 基因名称 | HRSP12 |
| Gene ID (NCBI) | 10247 |
| RRID | AB_2119169 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52758 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribonuclease UK114, also named HRSP12, is found predominantly in human adult kidney and liver and is responsible for inhibiting translation by cleaving mRNA. UK114 is mostly expressed in cytoplasm, but occasionally in nucleus in less differentiated cells. UK114 cleaves phosphodiester bonds only in single-stranded RNA. The levels of both the mRNA and protein are markedly reduced in heptatocellular tumors and in human hepatoma cell lines compared with normal liver tissues, suggesting that ribonuclease UK114 may be an important biomarker for heptatic carcinoma. Current antibody catalog# 12930-1-AP is a rabbit polyclonal antibody raised from human full-length UK114.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRSP12 antibody 12930-1-AP | Download protocol |
| IHC protocol for HRSP12 antibody 12930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Biol Y-box binding protein 1 and RNase UK114 mediate monocyte chemoattractant protein 1 mRNA stability in vascular smooth muscle cells. | ||
Genes Genomics P14.5 functions to inhibit cell migration and can be used as a prognostic marker in hepatocellular carcinoma |