IHCeasy HRSP12 Ready-To-Use IHC Kit
HRSP12 Ready-to-use reagent kit for IHC.
Reactivity
Human
Sample Type
FFPE tissue
Cat No : KHC0517
Synonyms
heat responsive protein 12, HRSP12, P14.5, PSP, Ribonuclease UK114, UK114, UK114 antigen homolog
Validation Data Gallery
Product Information
KHC0517 is a ready-to-use IHC kit for staining of HRSP12. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy HRSP12 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
HRSP12, also named ribonuclease UK114, is found predominantly in human adult kidney and liver and is responsible for inhibiting translation by cleaving mRNA. UK114 is mostly expressed in cytoplasm, but occasionally in nucleus in less differentiated cells. UK114 cleaves phosphodiester bonds only in single-stranded RNA. The levels of both the mRNA and protein are markedly reduced in heptatocellular tumors and in human hepatoma cell lines compared with normal liver tissues, suggesting that ribonuclease UK114 may be an important biomarker for heptatic carcinoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | heat responsive protein 12, HRSP12, P14.5, PSP, Ribonuclease UK114, UK114, UK114 antigen homolog |