验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse kidney tissue, human kidney tissue, Transfected HEK-293 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14192-1-AP targets HSD11B2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | HSD11B2 fusion protein Ag5146 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hydroxysteroid (11-beta) dehydrogenase 2 |
| Synonyms | 11-beta-hydroxysteroid dehydrogenase type II, 11-beta-hydroxysteroid dehydrogenase type 2, 11-beta-HSD2, 11-beta-HSD type II, 11 DH2 |
| Calculated Molecular Weight | 405 aa, 44 kDa |
| Observed Molecular Weight | 40-43 kDa |
| GenBank Accession Number | BC036780 |
| Gene Symbol | HSD11B2 |
| Gene ID (NCBI) | 3291 |
| RRID | AB_2119643 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P80365 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The HSD11B2 gene encodes the type II isoform of 11-beta-hydroxysteroid dehydrogenase, a microsomal enzyme complex responsible for the interconversion of biologically active cortisol and inactive cortisone. This protein catalyzes the cortisol-to-cortisone reaction. This protein expresses approximately 44 kDa in placenta, trophoblast, and distal colon, but in kidney tissue, there are two bands of approximately 44 and 48 kDa can be consistently observed and no signal is seen in decidua, adrenal, or liver(PMID:9048640). HSD11B2 protein was detected in CEM-C7 cells via immunoblot analysis as a dimer and trimer of approximately 80 and 120 kDa (PMID:23762608).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSD11B2 antibody 14192-1-AP | Download protocol |
| IHC protocol for HSD11B2 antibody 14192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nutrients Maternal High-Fructose Corn Syrup Intake Impairs Corticosterone Clearance by Reducing Renal 11β-Hsd2 Activity via miR-27a-Mediated Mechanism in Rat Offspring | ||
Int Immunopharmacol Mineralocorticoid promotes intestinal inflammation through receptor dependent IL17 production in ILC3s | ||
Oncotarget Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats. | ||
Ecotoxicol Environ Saf Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse. | ||
Carcinogenesis Modulation of the oral glucocorticoid system during black raspberry mediated oral cancer chemoprevention.
| ||
Sci Rep Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets. |
