• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67189-1-Ig (same clone as 67189-1-PBS)

    WB analysis using 67189-1-Ig (same clone as 67189-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67189-1-Ig (HSF1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

    IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSF1 antibody included
  • IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

    IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSF1 antibody included
  • WB analysis of HeLa using 67189-1-Ig (same clone as 67189-1-PBS)

    WB analysis of HeLa using 67189-1-Ig (same clone as 67189-1-PBS)

    WB result of HSF1 antibody (67189-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells. This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HSF1 Monoclonal antibody, PBS Only

HSF1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67189-1-PBS

产品说明书

CloneNo. 1F10A5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HSF1 Ready-To-Use IHC Kit. HSF1 primary antibody included.

产品信息

67189-1-PBS targets HSF1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 HSF1 fusion protein Ag29061 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock transcription factor 1
别名 Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1
计算分子量 529 aa, 57 kDa
观测分子量 68-80 kDa
GenBank蛋白编号BC014638
基因名称 HSF1
Gene ID (NCBI) 3297
RRIDAB_2882484
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ00613
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).