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HSF1 Monoclonal antibody

HSF1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67189-1-Ig

产品说明书

CloneNo. 1F10A5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat and More (1)

应用

WB, IHC, ELISA and More (1)

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HSF1 Ready-To-Use IHC Kit. HSF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67189-1-Ig targets HSF1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF, IHC
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原 HSF1 fusion protein Ag29061 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock transcription factor 1
别名 Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1
计算分子量 529 aa, 57 kDa
观测分子量 68-80 kDa
GenBank蛋白编号BC014638
基因名称 HSF1
Gene ID (NCBI) 3297
RRIDAB_2882484
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ00613
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

实验方案

Product Specific Protocols
WB protocol for HSF1 antibody 67189-1-IgDownload protocol
IHC protocol for HSF1 antibody 67189-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Pancreas

Heat Shock Factor 1 in Relation to Tumor Angiogenesis and Disease Progression in Patients With Pancreatic Cancer.

Authors - Qiuman Lin
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