验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67189 targets HSF1 in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | HSF1 fusion protein Ag29061 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | heat shock transcription factor 1 |
| 别名 | Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1 |
| 计算分子量 | 529 aa, 57 kDa |
| 观测分子量 | 68-80 kDa |
| GenBank蛋白编号 | BC014638 |
| 基因名称 | HSF1 |
| Gene ID (NCBI) | 3297 |
| RRID | AB_2919433 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q00613 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HSF1 antibody CL488-67189 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |