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验证数据展示

  • IF Staining of HepG2 using CL488-67189

    IF Staining of HepG2 using CL488-67189

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated HSF1 antibody (CL488-67189, Clone: 1F10A5 ) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HSF1 Monoclonal antibody

HSF1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67189

Print datasheet

CloneNo. 1F10A5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSF1 Ready-To-Use IHC Kit. HSF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67189-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67189 targets HSF1 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen HSF1 fusion protein Ag29061 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock transcription factor 1
Synonyms Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1
Calculated Molecular Weight 529 aa, 57 kDa
Observed Molecular Weight 68-80 kDa
GenBank Accession NumberBC014638
Gene Symbol HSF1
Gene ID (NCBI) 3297
RRIDAB_2919433
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ00613
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HSF1 antibody CL488-67189Download protocol
Standard Protocols
Click here to view our Standard Protocols