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验证数据展示

  • WB analysis of human testis using 84540-1-RR

    WB analysis of human testis using 84540-1-RR

    Human testis tissue were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 84540-1-RR

    IHC staining of mouse testis using 84540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84540-1-RR

    Affinity and Kinetic Characterization of 84540-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84540-1-RR against Human HSL were performed. The affinity constant is below 1 pM.

HSL Recombinant antibody

HSL Recombinant Antibody for WB, IHC, ELISA
Cat No. 84540-1-RR

产品说明书

CloneNo. 241875B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

LIPE, LHS, Hormone-sensitive lipase, EC:3.1.1.79, EC:3.1.1.23

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse adipose tissue, rat adipose tissue
Positive IHC detected inmouse testis tissue, mouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84540-1-RR targets HSL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HSL fusion protein Ag11360 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lipase, hormone-sensitive
别名 LIPE, LHS, Hormone-sensitive lipase, EC:3.1.1.79, EC:3.1.1.23
计算分子量 117 kDa
观测分子量84 kDa
GenBank蛋白编号BC070041
基因名称 HSL
Gene ID (NCBI) 3991
RRIDAB_3672045
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ05469
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.

实验方案

Product Specific Protocols
WB protocol for HSL antibody 84540-1-RRDownload protocol
IHC protocol for HSL antibody 84540-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols