验证数据展示
经过测试的应用
Positive WB detected in | human testis tissue, mouse adipose tissue, rat adipose tissue |
Positive IHC detected in | mouse testis tissue, mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84540-1-RR targets HSL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | HSL fusion protein Ag11360 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | lipase, hormone-sensitive |
Synonyms | LIPE, LHS, Hormone-sensitive lipase, EC:3.1.1.79, EC:3.1.1.23 |
Calculated Molecular Weight | 117 kDa |
Observed Molecular Weight | 84 kDa |
GenBank Accession Number | BC070041 |
Gene Symbol | HSL |
Gene ID (NCBI) | 3991 |
RRID | AB_3672045 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q05469 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for HSL antibody 84540-1-RR | Download protocol |
IHC protocol for HSL antibody 84540-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |