Human testis tissue were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

Sandwich ELISA standard curve of MP01374-1, Human HSL Recombinant Matched Antibody Pair - PBS only. 84540-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11360. 84540-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

The mean HSL concentration was determined to be 3.58 ng/mL in 3T3-L1 cell extract based on a 1.30 mg/mL extract load and 1.80 ng/mL in NIH/3T3 cell extract based on a 5.90 mg/mL extract load.

Cytometric bead array standard curve of MP01374-1, HSL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84540-3-PBS. Detection antibody: 84540-1-PBS. Standard: Ag11360. Range: 0.625-80 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 84540-1-RR against Human HSL were performed. The affinity constant is below 1 pM.