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验证数据展示

  • WB analysis using 66767-1-Ig (same clone as 66767-1-PBS)

    WB analysis using 66767-1-Ig (same clone as 66767-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66767-1-Ig (HSPB1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66767-1-Ig (same clone as 66767-1-PBS)

    IHC staining of human gliomas using 66767-1-Ig (same clone as 66767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66767-1-Ig (HSPB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPB1 antibody included
  • FC experiment of HepG2 using 66767-1-Ig (same clone as 66767-1-PBS)

    FC experiment of HepG2 using 66767-1-Ig (same clone as 66767-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (66767-1-Ig, Clone:3E7A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

  • IF Staining of zebrafish retina using 66767-1-Ig (same clone as 66767-1-PBS)

    IF Staining of zebrafish retina using 66767-1-Ig (same clone as 66767-1-PBS)

    Antibody staining on tissue sections of the zebrafish retina at 5 days post fertilisation (dpf) for Heatshock protein 27 (Hsp27;magenta); (#66767-1-Ig) Dilution 1:200. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66767-1-Ig (same clone as 66767-1-PBS)

    WB analysis of HeLa using 66767-1-Ig (same clone as 66767-1-PBS)

    WB result of HSP27 antibody (66767-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66767-1-Ig (same clone as 66767-1-PBS)

    IHC staining of human liver using 66767-1-Ig (same clone as 66767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66767-1-Ig (HSP27 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPB1 antibody included
  • Featured Product
  • KD/KO验证

HSP27 Monoclonal antibody, PBS Only

HSP27 Monoclonal Antibody for WB, IHC, IF, FC (Intra), Indirect ELISA
Cat No. 66767-1-PBS

产品说明书

CloneNo. 3E7A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, zebrafish

应用

WB, IHC, IF, FC (Intra), Indirect ELISA

别名

HSPB1, HSP28, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HSPB1 Ready-To-Use IHC Kit. HSPB1 primary antibody included.

产品信息

66767-1-PBS targets HSP27 in WB, IHC, IF, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB, IHC, IF, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, zebrafish
免疫原 HSP27 fusion protein Ag27859 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 27kDa protein 1
别名 HSPB1, HSP28, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein
计算分子量 19-23 kDa
观测分子量 27 kDa
GenBank蛋白编号BC012768
基因名称 HSPB1
Gene ID (NCBI) 3315
RRIDAB_2882113
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04792
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).