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  • Featured Product
  • KD/KO Validated

HSP27 Monoclonal antibody, PBS Only

HSP27 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E7A1

Cat No : 66767-1-PBS

Print datasheet

Synonyms

28 kDa heat shock protein, CMT2F, DKFZp586P1322, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HMN2B, HS.76067, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27

验证数据展示

  • WB analysis using 66767-1-Ig (same clone as 66767-1-PBS)

    WB analysis using 66767-1-Ig (same clone as 66767-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66767-1-Ig (HSPB1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66767-1-Ig (same clone as 66767-1-PBS)

    IHC staining of human gliomas using 66767-1-Ig (same clone as 66767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66767-1-Ig (HSPB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPB1 antibody included
  • IF Staining of zebrafish retina using 66767-1-Ig (same clone as 66767-1-PBS)

    IF Staining of zebrafish retina using 66767-1-Ig (same clone as 66767-1-PBS)

    Antibody staining on tissue sections of the zebrafish retina at 5 days post fertilisation (dpf) for Heatshock protein 27 (Hsp27;magenta); (#66767-1-Ig) Dilution 1:200. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66767-1-Ig (same clone as 66767-1-PBS)

    WB analysis of HeLa using 66767-1-Ig (same clone as 66767-1-PBS)

    WB result of HSP27 antibody (66767-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HSPB1 Ready-To-Use IHC Kit. HSPB1 primary antibody included.

产品信息

66767-1-PBS targets HSP27 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HSP27 fusion protein Ag27859 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 27kDa protein 1
Synonyms 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HMN2B, HS.76067, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27
Calculated Molecular Weight 19-23 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC012768
Gene Symbol HSPB1
Gene ID (NCBI) 3315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04792
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).