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- KD/KO Validated
HSP27 Monoclonal antibody
HSP27 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, zebrafish and More (1)
Applications
WB, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3E7A1
验证数据展示
经过测试的应用
Positive WB detected in | HCT 116 cells, HeLa cells, HSC-T6 cells, mouse liver tissue, U2OS cells, A549 cells, HepG2 cells, HuH-7 cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | zebrafish retina, MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 2 publications below |
产品信息
66767-1-Ig targets HSP27 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.
Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | Human, mouse, rat, zebrafish |
Cited Reactivity | human, mouse |
Immunogen | HSP27 fusion protein Ag27859 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | heat shock 27kDa protein 1 |
Synonyms | 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HMN2B, HS.76067, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27 |
Calculated Molecular Weight | 19-23 kDa |
Observed Molecular Weight | 27 kDa |
GenBank Accession Number | BC012768 |
Gene Symbol | HSPB1 |
Gene ID (NCBI) | 3315 |
RRID | AB_2882113 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04792 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HSP27 antibody 66767-1-Ig | Download protocol |
IHC protocol for HSP27 antibody 66767-1-Ig | Download protocol |
IF protocol for HSP27 antibody 66767-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncol Rep SUMOylation of HSP27 regulates PKM2 to promote esophageal squamous cell carcinoma progression. | ||