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验证数据展示

  • IF Staining of MCF-7 using CL594-66767

    IF Staining of MCF-7 using CL594-66767

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®594 HSP27 antibody (CL594-66767, Clone: 3E7A1 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated HSP27 Monoclonal antibody

HSP27 Monoclonal Antibody for IF/ICC
Cat No. CL594-66767

产品说明书

CloneNo. 3E7A1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

28 kDa heat shock protein, CMT2F, DKFZp586P1322, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HMN2B, HS.76067, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy HSPB1 Ready-To-Use IHC Kit. HSPB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66767-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66767 targets HSP27 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 HSP27 fusion protein Ag27859 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 27kDa protein 1
别名 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HMN2B, HS.76067, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27
计算分子量 19-23 kDa
观测分子量 27 kDa
GenBank蛋白编号BC012768
基因名称 HSPB1
Gene ID (NCBI) 3315
RRIDAB_2920032
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04792
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).

实验方案

Product Specific Protocols
IF protocol for CL594 HSP27 antibody CL594-66767Download protocol
Standard Protocols
Click here to view our Standard Protocols