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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated HSP47/SERPINH1 Polyclonal antibody

HSP47/SERPINH1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10875

Print datasheet

Synonyms

HSP47, SERPINH1, Cell proliferation-inducing gene 14 protein, CBP2, CBP1

验证数据展示

  • FC experiment of HepG2 using CL647-10875

    FC experiment of HepG2 using CL647-10875

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human HSP47 (CL647-10875) (red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10875-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10875 targets HSP47/SERPINH1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen HSP47/SERPINH1 fusion protein Ag1301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms HSP47, SERPINH1, Cell proliferation-inducing gene 14 protein, CBP2, CBP1
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC014623
Gene Symbol HSP47/SERPINH1
Gene ID (NCBI) 871
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50454
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 HSP47/SERPINH1 antibody CL647-10875Download protocol
Standard Protocols
Click here to view our Standard Protocols