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HSP47/SERPINH1 Recombinant antibody, PBS Only (Capture)

HSP47/SERPINH1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240752F2

Cat no : 83689-4-PBS

Print datasheet

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

验证数据展示

  • WB analysis using 83689-4-RR (same clone as 83689-4-PBS)

    WB analysis using 83689-4-RR (same clone as 83689-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83689-4-RR (HSP47/SERPINH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83689-4-RR (same clone as 83689-4-PBS)

    IHC staining of human ovarian cancer using 83689-4-RR (same clone as 83689-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83689-4-RR (HSP47/SERPINH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP47 antibody included
  • IF Staining of HeLa using 83689-4-RR (same clone as 83689-4-PBS)

    IF Staining of HeLa using 83689-4-RR (same clone as 83689-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47/SERPINH1 antibody (83689-4-RR, Clone: 240752F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83689-4-RR (same clone as 83689-4-PBS)

    IF Staining of HeLa using 83689-4-RR (same clone as 83689-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47/SERPINH1 antibody (83689-4-RR, Clone: 240752F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00677-2

    Sandwich ELISA standard curve of MP00677-2

    Sandwich ELISA standard curve of MP00677-2, Human HSP47/SERPINH1 Recombinant Matched Antibody Pair - PBS only. 83689-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1201. 83689-3-PBS was HRP conjugated as the detection antibody. Range: 0.23-15 ng/mL

  • Sample test of MP00677-2

    Sample test of MP00677-2

    HepG2, Hela and MCF-7 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Aliquots of the cell culture supernates were removed. Cells were lysed and assayed for human HSP47/SERPINH1. Human HSP47/SERPINH1 in HepG2 cell lysate measured 17.9 ng/mL. Human HSP47/SERPINH1 in Hela cell lysate measured 56.9 ng/mL. Human HSP47/SERPINH1 in MCF-7 cell lysate measured 35.1 ng/mL.

  • Affinity and Kinetic Characterization of 83689-4-RR

    Affinity and Kinetic Characterization of 83689-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83689-4-RR against Human HSP47/SERPINH1 were performed. The affinity constant is 5.99 nM.

Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.

产品信息

83689-4-PBS targets HSP47/SERPINH1 as part of a matched antibody pair:

MP00677-2: 83689-4-PBS capture and 83689-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms HSP47, SERPINH1, CBP2, CBP1, AsTP3
Calculated Molecular Weight46kDa
GenBank Accession NumberNM_001235.5
Gene Symbol HSP47/SERPINH1
Gene ID (NCBI) 871
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50454
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C