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- KD/KO Validated
HSPA2 Polyclonal antibody
HSPA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, human brain tissue, human kidney tissue, human skeletal muscle tissue, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse skeletal tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human normal colon, human ovary tumor tissue, human prostate hyperplasia tissue, human testis tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy HSPA2 Ready-To-Use IHC Kit. HSPA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12797-1-AP targets HSPA2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | HSPA2 fusion protein Ag3539 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | heat shock 70kDa protein 2 |
| Synonyms | HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2 |
| Calculated Molecular Weight | 639 aa, 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC036107 |
| Gene Symbol | HSPA2 |
| Gene ID (NCBI) | 3306 |
| RRID | AB_2119687 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54652 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA2 antibody 12797-1-AP | Download protocol |
| IHC protocol for HSPA2 antibody 12797-1-AP | Download protocol |
| IF protocol for HSPA2 antibody 12797-1-AP | Download protocol |
| IP protocol for HSPA2 antibody 12797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline. | ||
Cell Death Differ RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2.
| ||
Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. | ||
J Mol Cell Biol Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through cross talk between Ahr-Nrf2 pathway and MAPK signaling. | ||
Development Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes. | ||
Biochim Biophys Acta Mol Cell Res The human testis-enriched HSPA2 interacts with HIF-1α in epidermal keratinocytes, yet HIF-1α stability and HIF-1-dependent gene expression rely on the HSPA (HSP70) activity |
